Loading a ring: structure of the Bacillus subtilis DnaB protein, a co-loader of the replicative helicase.

@article{NnezRamrez2007LoadingAR,
  title={Loading a ring: structure of the Bacillus subtilis DnaB protein, a co-loader of the replicative helicase.},
  author={Rafael N{\'u}nez-Ram{\'i}rez and Marion Velten and Germ{\'a}n Tenorio Rivas and Patrice Polard and Jos{\'e} Mar{\'i}a Carazo and Luis Enrique Donate},
  journal={Journal of molecular biology},
  year={2007},
  volume={367 3},
  pages={764-9}
}
Loading of the ring-shaped replicative helicase is a critical step in the initiation of DNA replication. Bacillus subtilis has adopted a two-protein strategy to load its hexameric replicative helicase: DnaB and DnaI interact with the helicase and mediate its delivery onto DNA. We present here the 3D electron microscopy structure of the DnaB protein, along with a detailed analysis of both its oligomeric state and its domain organization. DnaB is organized as an asymmetric tetramer that is… CONTINUE READING

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