Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba.

  title={Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba.},
  author={F. Charri{\'e}re and N. Pavillon and T. Colomb and C. Depeursinge and T. Heger and E. Mitchell and P. Marquet and B. Rappaz},
  journal={Optics express},
  volume={14 16},
  • F. Charriére, N. Pavillon, +5 authors B. Rappaz
  • Published 2006
  • Materials Science, Medicine
  • Optics express
  • This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of pi, allowing to built 3-dimensional quantitative refractive index distributions by an inverse Radon transform. A 20x magnification allows a resolution better than 3 microm in all three dimensions, with accuracy better than 0.01 for the refractive index… CONTINUE READING
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