Liver tissue engineering based on aggregate assembly: efficient formation of endothelialized rat hepatocyte aggregates and their immobilization with biodegradable fibres.

@article{Pang2012LiverTE,
  title={Liver tissue engineering based on aggregate assembly: efficient formation of endothelialized rat hepatocyte aggregates and their immobilization with biodegradable fibres.},
  author={Yan Pang and Kevin Montagne and Marie Shinohara and Keiichiro Komori and Yasuyuki Sakai},
  journal={Biofabrication},
  year={2012},
  volume={4 4},
  pages={045004}
}
To realize long-term in vitro culture of hepatocytes at a high density while maintaining a high hepatic function for aggregate-based liver tissue engineering, we report here a novel culture method whereby endothelialized rat hepatocyte aggregates were formed using a PDMS microwell device and cultured in a perfusion bioreactor by introducing spacers between… CONTINUE READING