Liver cell calcium homeostasis in carbon tetrachloride liver cell injury: new data with fura2.

@article{Dolak1988LiverCC,
  title={Liver cell calcium homeostasis in carbon tetrachloride liver cell injury: new data with fura2.},
  author={James A. Dolak and Robert L. Waller and E. A. Glende and Richard O. Recknagel},
  journal={Journal of biochemical toxicology},
  year={1988},
  volume={3},
  pages={329-42}
}
The calcium fluorescent probe fura2 was used to measure concentration of free calcium in the cytosol of isolated rat hepatocytes in suspension. The resting level in untreated hepatocytes was 121 nM. On addition of CCl4 at a concentration of 0.5 mM, cytosolic free calcium rose sharply and reached a statistically significant (P less than 0.05) steady plateau level of about 190 nM within five minutes. With a concentration of 1.0 mM CCl4, cytosolic free calcium rose within ten minutes to a plateau… CONTINUE READING