Live spheroid formation recorded with light sheet-based fluorescence microscopy.

Abstract

We provide a detailed protocol for a three-dimensional long-term live imaging of cellular spheroids with light sheet-based fluorescence microscopy. The protocol allows the recording of all phases of spheroid formation in three dimensions, including cell proliferation, aggregation, and compaction. We employ the human hepatic cell line HepaRG transfected with the fusion protein H2B-GFP, i.e., a fluorescing histone. The protocol allows monitoring the effect of drugs or toxicants.

DOI: 10.1007/978-1-4939-2080-8_3

Cite this paper

@article{Pampaloni2015LiveSF, title={Live spheroid formation recorded with light sheet-based fluorescence microscopy.}, author={F. Pampaloni and Roli Richa and N. Ansari and Ernst H. K. Stelzer}, journal={Methods in molecular biology}, year={2015}, volume={1251}, pages={43-57} }