Live dynamic imaging of caveolae pumping targeted antibody rapidly and specifically across endothelium in the lung

@article{Oh2007LiveDI,
  title={Live dynamic imaging of caveolae pumping targeted antibody rapidly and specifically across endothelium in the lung},
  author={Phil Oh and Per Henrik Borgstr{\"o}m and Halina Witkiewicz and Yan Li and Bengt J. Borgstr{\"o}m and Adrian Chrastina and Koji Iwata and Kurt R. Zinn and Richard Baldwin and Jacqueline E. Testa and Jan E. Schnitzer},
  journal={Nature Biotechnology},
  year={2007},
  volume={25},
  pages={327-337}
}
How effectively and quickly endothelial caveolae can transcytose in vivo is unknown, yet critical for understanding their function and potential clinical utility. Here we use quantitative proteomics to identify aminopeptidase P (APP) concentrated in caveolae of lung endothelium. Electron microscopy confirms this and shows that APP antibody targets nanoparticles to caveolae. Dynamic intravital fluorescence microscopy reveals that targeted caveolae operate effectively as pumps, moving antibody… CONTINUE READING