Live-cell nucleocytoplasmic protein shuttle assay utilizing laser confocal microscopy and FRAP.

@article{Howell2002LivecellNP,
  title={Live-cell nucleocytoplasmic protein shuttle assay utilizing laser confocal microscopy and FRAP.},
  author={Jenny L Howell and Ray Truant},
  journal={BioTechniques},
  year={2002},
  volume={32 1},
  pages={
          80-2, 84, 86-7
        }
}
In eukaryotes, protein trafficking to and from the nucleus, or shuttling, has been demonstrated to be an important function for proteins that have vital roles in one or both subcellular compartments. Current techniques of detecting protein nuclear shuttling are extremely labor intensive and only statically visualize evidence of shuttling. Fluorescence recovery after photobleaching (FRAP), or fluorescence microphotolysis, has proven to be an effective method of analyzing protein dynamics in live… CONTINUE READING
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