Live-cell imaging reveals divergent intracellular dynamics of polyglutamine disease proteins and supports a sequestration model of pathogenesis.

@article{Chai2002LivecellIR,
  title={Live-cell imaging reveals divergent intracellular dynamics of polyglutamine disease proteins and supports a sequestration model of pathogenesis.},
  author={Yaohui Chai and Jianqiang Shao and Victor M. Miller and Aislinn Joanmarie Williams and Henry L. Paulson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 14},
  pages={9310-5}
}
Protein misfolding and aggregation are central features of the polyglutamine neurodegenerative disorders, but the dynamic properties of expanded polyglutamine proteins are poorly understood. Here, we use fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) with green fluorescent protein fusion proteins to study… CONTINUE READING