Live cell imaging of phosphoinositides with expressed inositide binding protein domains.

Abstract

Inositol lipids and calcium signaling has been inseparable twins during the 1980s when the molecular details of phospholipase C-mediated generation of inositol 1,4,5-trisphosphate (InsP3) and its Ca2+ mobilizing action were discovered. Since then, both the Ca2+ and inositol lipid signaling fields have hugely expanded and the tools allowing dissection of the… (More)
DOI: 10.1016/j.ymeth.2008.09.019

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