Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9

  title={Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9},
  author={Peiwu Qin and Mahmut Parlak and Cem Kuscu and Jigar N. Bandaria and Mustafa Mir and Karol Szlachta and Ritambhara Singh and Xavier Darzacq and Ahmet Yildiz and Mazhar Adli},
  booktitle={Nature communications},
Imaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for imaging of chromatin within live cells. However, these methods are mostly applicable to highly repetitive regions, whereas imaging regions with low or no repeats remains as a challenge. To address this challenge, we design single-guide RNAs (sgRNAs) integrated with up to 16 MS2 binding motifs to enable robust… CONTINUE READING
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