Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

@inproceedings{Wei2014LivecellIO,
  title={Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering},
  author={Lu Wei and Fanghao Hu and Yihui Shen and Zhixing Chen and Y Eugene Yu and C. J. Lin and Meng C Wang and Wei Min},
  booktitle={Nature Methods},
  year={2014}
}
Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.