Live cell fluorescence microscopy to study microbial pathogenesis
@article{Hoppe2009LiveCF, title={Live cell fluorescence microscopy to study microbial pathogenesis}, author={Adam D. Hoppe and Stephanie Seveau and Joel A. Swanson}, journal={Cellular Microbiology}, year={2009}, volume={11} }
Advances in microscopy and fluorescent probes provide new insight into the nanometer‐scale biochemistry governing the interactions between eukaryotic cells and pathogens. When combined with mathematical modelling, these new technologies hold the promise of qualitative, quantitative and predictive descriptions of these pathways. Using the light microscope to study the spatial and temporal relationships between pathogens, host cells and their respective biochemical machinery requires an…
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References
SHOWING 1-10 OF 57 REFERENCES
Roundtrip explorations of bacterial infection: from single cells to the entire host and back.
- BiologyTrends in microbiology
- 2007
Progression of bacterial infections studied in real time – novel perspectives provided by multiphoton microscopy
- BiologyCellular microbiology
- 2007
The current advancements and knowledge in multiphoton microscopy and how it can be related to the field of microbial infections are detailed.
Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells
- Biology, ChemistryNature Methods
- 2004
Both theoretical and experimental approaches that discriminate the parallel versus the sequential energy transfer processes in the 3-FRET system are described, which highlight the potential of 3- FRET microscopy in studies of spatial and temporal regulation of signaling processes in living cells.
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics
- BiologyNature Methods
- 2008
By allowing observation of a wide variety of nanoscale dynamics, live-cell PALM provides insights into molecular assembly during the initiation, maturation and dissolution of cellular processes.
A guide to accurate fluorescence microscopy colocalization measurements.
- BiologyBiophysical journal
- 2006
Through simulations, imaging of fluorescent antibodies adsorbed on glass and cell measurements, it is shown that ICCS performs much better than standard colocalization algorithms at moderate to high densities of particles, which are often encountered in cellular systems.
Three-dimensional FRET reconstruction microscopy for analysis of dynamic molecular interactions in live cells.
- BiologyBiophysical journal
- 2008
These results verify previous observations that Cdc42 signaling is localized to the advancing margins of forming phagosomes in macrophages and devise and validate a new approach (called 3D-FRET stoichiometry reconstruction, 3DFSR), which provides orders of magnitude gains in both sensitivity and accuracy.
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
- BiologyScience
- 2004
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination…
Technoreview: Molecular imaging of host–pathogen interactions in intact small animals
- BiologyCellular microbiology
- 2004
These new instruments, when used in conjunction with targeted contrast agents, reporter substrates and radiopharmaceuticals, enable ‘molecular imaging’ with enormous potential for elucidating host–pathogen interactions in intact animal models.
Evolutionary optimization of fluorescent proteins for intracellular FRET
- Biology, ChemistryNature Biotechnology
- 2005
A quantitative evolutionary strategy using fluorescence-activated cell sorting to optimize a cyan-yellow fluorescent protein pair for FRET provides substantially improved sensitivity and dynamic range for a broad range of molecular imaging and screening applications.
Tracking single Kinesin molecules in the cytoplasm of mammalian cells.
- BiologyBiophysical journal
- 2007
By combining direct genetic labeling and single molecule imaging in vivo, this work establishes an important new biophysical method for observing single molecules expressed and localized in the mammalian cytoplasm.