Live-cell analysis of a green fluorescent protein-tagged herpes simplex virus infection.

@article{Elliott1999LivecellAO,
  title={Live-cell analysis of a green fluorescent protein-tagged herpes simplex virus infection.},
  author={Gillian Elliott and Peter G O'Hare},
  journal={Journal of virology},
  year={1999},
  volume={73 5},
  pages={4110-9}
}
Many stages of the herpes simplex virus maturation pathway have not yet been defined. In particular, little is known about the assembly of the virion tegument compartment and its subsequent incorporation into maturing virus particles. Here we describe the construction of a herpes simplex virus type 1 (HSV-1) recombinant in which we have replaced the gene encoding a major tegument protein, VP22, with a gene expressing a green fluorescent protein (GFP)-VP22 fusion protein (GFP-22). We show that… CONTINUE READING

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