Live-cell 3D super-resolution imaging in thick biological samples

  title={Live-cell 3D super-resolution imaging in thick biological samples},
  author={Francesca Cella Zanacchi and Zeno Lavagnino and Michela Perrone Donnorso and A. Del Bue and Laura Furia and Mario Faretta and Alberto Diaspro},
  journal={Nature Methods},
We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50–150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids. 
Recent Discussions
This paper has been referenced on Twitter 26 times over the past 90 days. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-10 of 100 extracted citations

Similar Papers

Loading similar papers…