Live birth after intrauterine insemination with spermatozoa from an oligoasthenozoospermic patient vitrified without permeable cryoprotectants.

@article{Snchez2012LiveBA,
  title={Live birth after intrauterine insemination with spermatozoa from an oligoasthenozoospermic patient vitrified without permeable cryoprotectants.},
  author={R Corripio S{\'a}nchez and Vladimir Isachenko and Anna M. Petrunkina and Jennie Risopatr{\'o}n and Mabel Schulz and Evgenia Isachenko},
  journal={Journal of andrology},
  year={2012},
  volume={33 4},
  pages={559-62}
}
We report the first case of a healthy baby born after intrauterine insemination with vitrified swim-up spermatozoa from an oligoasthenozoospermic patient. A 39-year-old patient was subjected to intrauterine insemination with spermatozoa from her 35-year-old husband, diagnosed with oligoasthenozoospermia. The swim-up spermatozoa from 2 ejaculates were suspended in a culture medium supplemented with 1% human serum albumin and 0.25 M sucrose. Three hermetically packaged 100-μL sperm portions (each… CONTINUE READING