Live Cell Monitoring of hiPSC Generation and Differentiation Using Differential Expression of Endogenous microRNAs

@inproceedings{Kamata2010LiveCM,
  title={Live Cell Monitoring of hiPSC Generation and Differentiation Using Differential Expression of Endogenous microRNAs},
  author={Masakazu Kamata and Min Liang and Shirley X.L. Liu and Yoshiko Nagaoka and Irvin S. Y. Chen},
  booktitle={PloS one},
  year={2010}
}
Human induced pluripotent stem cells (hiPSCs) provide new possibilities for regenerative therapies. In order for this potential to be achieved, it is critical to efficiently monitor the differentiation of these hiPSCs into specific lineages. Here, we describe a lentiviral reporter vector sensitive to specific microRNAs (miRNA) to show that a single vector bearing multiple miRNA target sequences conjugated to different reporters can be used to monitor hiPSC formation and subsequent… CONTINUE READING

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Generation of human induced pluripotent stem cells from dermal fibroblasts.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2008
VIEW 4 EXCERPTS
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