Liquid chromatography-tandem mass spectrometry determination of loperamide and its main metabolite desmethylloperamide in biological specimens and application to forensic cases.

@article{Johansen2004LiquidCM,
  title={Liquid chromatography-tandem mass spectrometry determination of loperamide and its main metabolite desmethylloperamide in biological specimens and application to forensic cases.},
  author={Sys Stybe Johansen and Jytte Lundsby Jensen},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2004},
  volume={811 1},
  pages={31-6}
}
A liquid chromatographic mass spectrometric (LC/MS/MS) method has been developed for the determination of loperamide in whole blood and other biological specimens. The procedure involves liquid-liquid extraction of loperamide, desmethylloperamide and methadone-D3 (internal standard) with butyl acetate. Confirmation and quantification was done by positive electrospray ionisation with a triple quadrupole mass spectrometer operating in multiple reaction-monitoring (MRM) mode. Two MRM transitions… CONTINUE READING