Liquid chromatography-tandem mass spectrometry method for the simultaneous determination of delta-ALA, tyrosine and creatinine in biological fluids.

@article{Felitsyn2004LiquidCM,
  title={Liquid chromatography-tandem mass spectrometry method for the simultaneous determination of delta-ALA, tyrosine and creatinine in biological fluids.},
  author={Natalia M Felitsyn and George N Henderson and Margaret O. James and Peter W. Stacpoole},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2004},
  volume={350 1-2},
  pages={219-30}
}
BACKGROUND Several acquired and congenital human disorders perturb the concentrations of delta-aminolevulinate (delta-ALA), creatinine and tyrosine in biological fluids. There is currently no facile, sensitive and specific method to measure these analytes simultaneously. METHOD We developed an LC-MS/MS method to quantify delta-ALA, creatinine and tyrosine in urine that requires minimal sample preparation and no derivatization. The method is also applicable to the analysis of tyrosine in… CONTINUE READING