Liquid chromatography high-resolution mass spectrometry for fatty acid profiling.

@article{Bromke2015LiquidCH,
  title={Liquid chromatography high-resolution mass spectrometry for fatty acid profiling.},
  author={Mariusz A. Bromke and Anton Hochmuth and Takayuki Tohge and Alisdair R. Fernie and Patrick Giavalisco and Asdrubal Burgos and Lothar Willmitzer and Yariv Brotman},
  journal={The Plant journal : for cell and molecular biology},
  year={2015},
  volume={81 3},
  pages={529-36}
}
Quantification of fatty acids has been crucial to elucidate lipid biosynthesis pathways in plants. To date, fatty acid identification and quantification has relied mainly on gas chromatography (GC) coupled to flame ionization detection (FID) or mass spectrometry (MS), which requires the derivatization of samples and the use of chemical standards for annotation. Here we present an alternative method based on a simple procedure for the hydrolysis of lipids, so that fatty acids can be quantified… CONTINUE READING
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