Liquid chromatography for iothalamate in biological samples.

  title={Liquid chromatography for iothalamate in biological samples.},
  author={Rajiv L Agarwal and Nina Vasavada and Shawn D Chase},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  volume={785 2},
We have previously reported an iothalamate assay for the assessment of the glomerular filtration rate (GFR) that required a long column equilibration time and 22 min run time per sample. We now report a simpler assay that requires a run time of only 5.5 min and is more precise and accurate than the earlier technique. The mobile phase consisted of methanol-acetonitrile-50 mM sodium monobasic phosphate (10:5:85, v/v) at pH 4.4, pumped at a rate of 1.5 ml/min on a C(18) reversed-phase column… CONTINUE READING
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