Liquid chromatography and mass spectrometry with post-column partial reduction for the analysis of native and scrambled disulfide bonds.

@article{Li2013LiquidCA,
  title={Liquid chromatography and mass spectrometry with post-column partial reduction for the analysis of native and scrambled disulfide bonds.},
  author={Xiaojuan Li and Wei Xu and Brittany Paporello and Daisy Richardson and Hongcheng Liu},
  journal={Analytical biochemistry},
  year={2013},
  volume={439 2},
  pages={184-6}
}
A method capable of detecting both native and scrambled disulfide bonds has been established. Nonreduced protein digests were separated using a reversed-phase C18 column, partially reduced by post-column addition of a reducing reagent, and then analyzed by mass spectrometry. Disulfide bond linkage was established by matching the retention times of cysteine… CONTINUE READING