Liquid chromatographic-tandem mass spectrometric method for the quantitation of huperzine A in dog plasma.

@article{Wang2004LiquidCM,
  title={Liquid chromatographic-tandem mass spectrometric method for the quantitation of huperzine A in dog plasma.},
  author={Yingwu Wang and Dafeng Chu and Jingkai Gu and John Paul Fawcett and Yi Wu and Wan-hui Liu},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2004},
  volume={803 2},
  pages={375-8}
}
A rapid and sensitive LC-MS-MS method for the determination of huperzine A in dog plasma using huperzine B as internal standard has been developed and validated. The analyte and internal standard were extracted from plasma using n-hexane-dichloromethane-2-propanol (300:150:15, v/v/v), chromatographed on a C(18) column (5 microm, 50 mm x 4.6 mm i.d.) with a mobile phase consisting of acetonitrile-methanol-10mM ammonium acetate (35:40:25, v/v/v), and detected using a tandem mass spectrometer with… CONTINUE READING