Liquid chromatographic assay of tylosin in animal feeds.

@article{Houglum1996LiquidCA,
  title={Liquid chromatographic assay of tylosin in animal feeds.},
  author={Joel E. Houglum and Martin Tasler},
  journal={Journal of AOAC International},
  year={1996},
  volume={79 2},
  pages={369-74}
}
A liquid chromatographic (LC) method is described for the assay of tylosin (tylosin A, tylosin B, and tylosin-urea adduct [TUA]) in animal feeds at approximately 4-200 g/ton. Samples are extracted with an equal mixture of methanol and 0.1M pH 8 phosphate buffer. An acidic alumina column cleanup step is used prior to reversed-phase LC. Determination is… CONTINUE READING