Liquid chromatographic and electrophoretic characterisation of extracellular beta-glucosidase of Pleurotus ostreatus grown in organic waste.

@article{Morais2002LiquidCA,
  title={Liquid chromatographic and electrophoretic characterisation of extracellular beta-glucosidase of Pleurotus ostreatus grown in organic waste.},
  author={Helena Morais and Cristina Ramos and Natalia Matos and Esther Forg{\'a}cs and Tibor Cserh{\'a}ti and Vitor C Almeida and Jos{\'e} Renato Assis Lemos Marques de Oliveira and Yassin Darwish and Zolt{\'a}n Il{\'e}s},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2002},
  volume={770 1-2},
  pages={111-9}
}
The production of beta-glucosidase by the ligninolytic fungus Pleurotus ostreatus has been studied in different culture media containing agro-industrial wastes. The enzyme is purified by anion-exchange chromatography, the molecular mass and isoelectric point of purified beta-glucosidase are measured by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing and the stability and kinetic parameters of the enzyme assessed by spectrophotometry. It has been… CONTINUE READING

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