Liquid chromatographic and capillary electrophoretic examination of intact and degraded fusion protein CTLA4Ig and kinetics of conformational transition.

@article{Greve1996LiquidCA,
  title={Liquid chromatographic and capillary electrophoretic examination of intact and degraded fusion protein CTLA4Ig and kinetics of conformational transition.},
  author={Kari Greve and D. E. Peter Hughes and Priscilla Richberg and Michael Kats and Barry L. Karger},
  journal={Journal of chromatography. A},
  year={1996},
  volume={723 2},
  pages={
          273-84
        }
}
Methods have been developed for the CE and HPLC analysis of CTLA4Ig, an immunoglobulin fusion protein. Two different LC approaches, size-exclusion (SEC) and "mixed-mode" ion-exchange (ABx), were developed along with a CE method that uses a micellar electrokinetic chromatographic buffer consisting of borate ions, sodium dodecyl sulfate and acetonitrile. These assays measure the presence of several CTLA4Ig-related species, and the observed changes resulting from multiple modes of degradation. In… CONTINUE READING

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