Liquid biopsy: monitoring cancer-genetics in the blood

@article{Crowley2013LiquidBM,
  title={Liquid biopsy: monitoring cancer-genetics in the blood},
  author={Emily Crowley and Federica Di Nicolantonio and Fotios Loupakis and Alberto Bardelli},
  journal={Nature Reviews Clinical Oncology},
  year={2013},
  volume={10},
  pages={472-484}
}
Cancer is associated with mutated genes, and analysis of tumour-linked genetic alterations is increasingly used for diagnostic, prognostic and treatment purposes. The genetic profile of solid tumours is currently obtained from surgical or biopsy specimens; however, the latter procedure cannot always be performed routinely owing to its invasive nature. Information acquired from a single biopsy provides a spatially and temporally limited snap-shot of a tumour and might fail to reflect its… 
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References

SHOWING 1-10 OF 190 REFERENCES
Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
TLDR
Serial analysis of cancer genomes in plasma constitutes a new paradigm for the study of clonal evolution in human cancers, establishing proof of principle that exome-wide analysis of circulating tumour DNA could complement current invasive biopsy approaches to identify mutations associated with acquired drug resistance in advanced cancers.
Multi-Purpose Utility of Circulating Plasma DNA Testing in Patients with Advanced Cancers
Tumor genomic instability and selective treatment pressures result in clonal disease evolution; molecular stratification for molecularly targeted drug administration requires repeated access to tumor…
Circulating mutant DNA to assess tumor dynamics
TLDR
It is found that ctDNA measurements could be used to reliably monitor tumor dynamics in subjects with cancer who were undergoing surgery or chemotherapy, and it is suggested that this personalized genetic approach could be generally applied to individuals with other types of cancer.
Tumor-associated copy number changes in the circulation of patients with prostate cancer identified through whole-genome sequencing
TLDR
The genomic landscape of prostate cancer can be established by non-invasive means from plasma DNA by developing an approach based on a benchtop high-throughput platform, that is, Illuminas MiSeq instrument.
Circulating tumour markers can define patients with normal colons, benign polyps and cancers
TLDR
The best three marker DNA model was able to discriminate the normal population from populations with adenoma and carcinoma with a ROC curve of 0.810, which offers the prospect of a simple blood test to screen for colorectal cancers and polyps.
Genomic analysis of circulating cell-free DNA infers breast cancer dormancy.
TLDR
The potential of SNP/CNV analysis of cfDNA to distinguish between patients with breast cancer and healthy controls during routine follow-up is demonstrated, and specific CNVs were detected in cfDNA, mirroring the primary tumor, up to 12 yr after diagnosis despite no other evidence of disease.
Noninvasive Identification and Monitoring of Cancer Mutations by Targeted Deep Sequencing of Plasma DNA
TLDR
Levels of mutant alleles reflected the clinical course of the disease and its treatment—for example, stabilized disease was associated with low allelic frequency, whereas patients at relapse exhibited a rise in frequency, and TAm-Seq will need to achieve a more sensitive detection limit to identify mutations in the plasma of patients with less advanced cancers.
Monitoring of circulating tumour-associated DNA as a prognostic tool for oral squamous cell carcinoma
TLDR
The results suggest that the assessment of microsatellite status in the serum DNA could be a useful predictive tool to monitor disease prognosis.
Detection of Chromosomal Alterations in the Circulation of Cancer Patients with Whole-Genome Sequencing
TLDR
An approach to directly identify tumor-derived chromosomal alterations through analysis of circulating cell-free DNA from cancer patients is described and represents a useful method for the noninvasive detection of human tumors that is not dependent on the availability of tumor biopsies.
Cancer genome scanning in plasma: detection of tumor-associated copy number aberrations, single-nucleotide variants, and tumoral heterogeneity by massively parallel sequencing.
TLDR
This study explored the use of shotgun massively parallel sequencing of plasma DNA from cancer patients to scan a cancer genome noninvasively and showed that plasma DNA sequencing is a valuable approach for studying tumoral heterogeneity.
...
...