Lipoxygenase mRNA in rabbit reticulocytes. Its isolation, characterization and translational repression.

@article{Thiele1982LipoxygenaseMI,
  title={Lipoxygenase mRNA in rabbit reticulocytes. Its isolation, characterization and translational repression.},
  author={Bernd J. Thiele and H H Andree and Matthias H{\"o}hne and Svetlana Rapoport},
  journal={European journal of biochemistry},
  year={1982},
  volume={129 1},
  pages={133-41}
}
The synthesis of the erythroid lipoxygenase, an enzyme which is of importance for the degradation of mitochondria during the maturation of reticulocytes to erythrocytes, was studied in reticulocytes from bone marrow and in density-separated fractions from peripheral blood of anemic rabbits. Lipoxygenase mRNA was enriched to about 75% by digestion of polysomes with protease K, poly(U)-Sepharose chromatography and repeated sucrose gradient centrifugation. From sucrose gradient centrifugation… CONTINUE READING