Lipoxygenase mRNA Silencing in Erythroid Differentiation The 3′UTR Regulatory Complex Controls 60S Ribosomal Subunit Joining

Abstract

15-lipoxygenase (LOX) expression is translationally silenced in early erythroid precursor cells by a specific mRNA-protein complex formed between the differentiation control element in the 3' untranslated region (UTR) and hnRNPs K and E1. The 3'UTR regulatory complex prevents translation initiation by an unknown mechanism. We demonstrate that the 40S ribosomal subunit can be recruited and scan to the translation initiation codon even when the silencing complex is bound to the 3'UTR. However, the joining of the 60S ribosomal subunit at the AUG codon to form a translation competent 80S ribosome is inhibited, unless initiation is mediated by the IGR-IRES of the cricket paralysis virus. These findings identify the critical step at which LOX mRNA translation is controlled and reveal that 60S subunit joining can be specifically regulated.

DOI: 10.1016/S0092-8674(01)00212-4

Extracted Key Phrases

6 Figures and Tables

0204060'02'04'06'08'10'12'14'16
Citations per Year

405 Citations

Semantic Scholar estimates that this publication has 405 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Ostareck2001LipoxygenaseMS, title={Lipoxygenase mRNA Silencing in Erythroid Differentiation The 3′UTR Regulatory Complex Controls 60S Ribosomal Subunit Joining}, author={Dirk H. Ostareck and Antje Ostareck-Lederer and Ivan N Shatsky and Matthias W. Hentze}, journal={Cell}, year={2001}, volume={104}, pages={281-290} }