Lipoxygenase-like enzyme in rat testis microsomes.

  title={Lipoxygenase-like enzyme in rat testis microsomes.},
  author={I Shahin and Shlomo Grossman and Benjamin Sredni},
  journal={Biochimica et biophysica acta},
  volume={529 2},
Microsomes, separated from rat testes, were found capable of oxidizing linoleate and arachidonate. The enzyme activity was solubilized with 1% Triton X-100 in acetate buffer (pH 5.0) and purified by affinity chromatography. The overall purification from the starting preparation was approx. 40-fold. The affinity-purified enzyme was almost homogeneous as… CONTINUE READING