Lipoxygenase: a new pathway for 2-aminofluorene bioactivation.

  title={Lipoxygenase: a new pathway for 2-aminofluorene bioactivation.},
  author={Sandip Kumar Roy and Arun P. Kulkarni},
  journal={Cancer letters},
  volume={60 1},
Soybean lipoxygenase mediated co-oxidation of 2-aminofluorene (2-AF), a putative carcinogen and teratogen, was examined. 2-AF metabolism was followed spectrally as a decrease in absorbance at 286 nm. The enzymatic oxidation displayed a pH optimum at 9.5 with a Km of 0.27 mM and specific activity of 521.7 +/- 46.6 nmol/min per nmol of enzyme. The generation… CONTINUE READING