Liposome-mediated transfection of fetal lung epithelial cells: DNA degradation and enhanced superoxide toxicity.

@article{Tanswell1998LiposomemediatedTO,
  title={Liposome-mediated transfection of fetal lung epithelial cells: DNA degradation and enhanced superoxide toxicity.},
  author={Alan Keith Tanswell and Olivier Staub and Richard Iles and Rosetta Belcastro and Judy Cabacungan and Larisa Sedlackova and Brent M. Steer and Yanxia Wen and Jim Hu and Hugh O'brodovich},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  year={1998},
  volume={275 3},
  pages={L452-L460}
}
Cationic liposomes, 1:1 (mol/mol) 1,2-dioleoyldimethylammonium chloride-1,2-dioleoyl- sn-glycero-3-phosphoethanolamine, were used to transfect primary cultures of distal rat fetal lung epithelial cells with pCMV4-based plasmids. A DNA-to-lipid ratio of 1:10 to 1:15 (wt/wt) optimized DNA uptake over a 24-h exposure. At a fixed DNA-to-lipid ratio of 1:15, chloramphenicol acetyltransferase (CAT) reporter gene expression declined at lipid concentrations > 2.5 nmol/cm2 cell surface area, whereas DNA… CONTINUE READING

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The cytosol constitutes a metabolic barrier against transgene delivery to the nucleus (Abstract)

  • D. Lechardeur, K.-J. Sohn, B. Beatty, J. Squire, H. O’Brodovich, G. Lukacs
  • Pediatr. Pulmonol
  • 1997
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