Liposome-mediated DNA uptake and transient expression in Thermotoga

  title={Liposome-mediated DNA uptake and transient expression in Thermotoga},
  author={J Yu and Madeline Vargas and Christina Mityas and Kenneth M. Noll},
We report here the successful application of a PCR-based method to detect genetic transformation of Thermotoga neapolitana and Thermotoga maritima. Plasmid vectors were constructed using pRQ7, an 846-bp plasmid found in Thermotoga species strain RQ7, which replicates by a rolling circle mechanism. The vector pJY1 was constructed by placing a gene encoding a thermostable chloramphenicol acetyltransferase from Staphylococcus aureus under the control of the tac promoter and joining this with pRQ7… CONTINUE READING


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