• DOI:10.5414/CPXCES13EA03
• Corpus ID: 11388057

Liposomal cisplatin can overcome chemotherapy resistance of A2780 ovarian cancer cells by inducing the extrinsic apoptotic pathway.

@article{Stlting2014LiposomalCC,
  title={Liposomal cisplatin can overcome chemotherapy resistance of A2780 ovarian cancer cells by inducing the extrinsic apoptotic pathway.},
  author={Daniel Philipp St{\"o}lting and Martin Koch and Michael Wiese and Hans Dieter Royer and Gerd Bendas},
  journal={International journal of clinical pharmacology and therapeutics},
  year={2014},
  volume={52 1},
  pages={
          78-81
        }
}

• D. Stölting, Martin Koch, +2 authors G. Bendas
• Published 2014
• Biology
• International journal of clinical pharmacology and therapeutics

Cisplatin (CDDP) is a principal compo­ nent of standard ovarian cancer chemother­ apy. However, the frequent development of cellular resistance to CDDP severely restricts the clinical therapeutic success. The molecu­ lar mechanisms of resistance are manifold and not completely understood [1]. Previously, we reported that liposo­ mal CDDP overcomes CDDP resistance of A2780cis human ovarian cancer cells [2]. However, the superior cytotoxic activi­ ty of liposomal CDDP vs. the free drug in A2780cis cells did not correlate with DNA platination levels, thus suggesting a different mode of action of liposomal CDDP com­ pared to free CDDP. To gain an insight into liposomal CDDP activity, a transcriptome analysis of A2780cis cells, either untreated, or in response to ex­ posure with IC50 values of free or liposomal CDDP has been performed [3]. A process network analysis of up­regulated genes indi­ cated that liposomal CDDP induced a highly different gene expression profile compared to the free drug. p53 was identified as play­ ing a key role in directing transcriptional responses to free or liposomal CDDP. Most interestingly, the transcriptome data showed that liposomal CDDP, in contrast to the free drug, induces expression of genes from the extrinsic pathway of apoptosis, which indi­ cates how liposomal CDDP can overcome chemoresistance of A2780cis cells. The aim of this study was to gain further insight into liposomal CDDP activity and to confirm prior findings on apoptosis induction on a protein and functional level. Materials and methods