Lipopolysaccharides possessing two L-glycero-D-manno-heptopyranosyl-alpha -(1-->5)-3-deoxy-D-manno-oct-2-ulopyranosonic acid moieties in the core region. The structure of the core region of the lipopolysaccharides from Burkholderia caryophylli.

Abstract

The carbohydrate backbone of the core-lipid A region was characterized from the lipopolysaccharides (LPSs) of the plant-pathogenic bacterium Burkholderia caryophylli. For the first time, the presence of two moieties of l-glycero-d-manno-heptopyranosyl-alpha-(1-->5)-3-deoxy-d-manno-oct-2-ulopyranosonic acid was identified in a core region, which is of particular interest with regard to the biosynthesis of this and of LPSs in general. The LPSs of B. caryophylli were degraded by mild hydrazinolysis (de-O-acylation), treatment with 48% aqueous HF at 4 degrees C (cleavage of phosphate groups and destruction of the O-specific polysaccharides), reduction with NaBH4, and de-N-acylation utilizing hot KOH. The major oligosaccharide representing the carbohydrate backbone of the core region and lipid A was isolated by high-performance anion-exchange chromatography. Its analysis employing compositional and methylation analyses, matrix-assisted laser desorption/ionization mass spectrometry, and (1)H and (13)C NMR spectroscopy applying various one-dimensional and two-dimensional experiments identified the following structure. Structure I All sugars are pyranoses and alpha-linked, if not stated otherwise. Hep is l-glycero-d-manno-heptose, Kdo is 3-deoxy-d-manno-oct-2-ulosonic acid.

Cite this paper

@article{Molinaro2002LipopolysaccharidesPT, title={Lipopolysaccharides possessing two L-glycero-D-manno-heptopyranosyl-alpha -(1-->5)-3-deoxy-D-manno-oct-2-ulopyranosonic acid moieties in the core region. The structure of the core region of the lipopolysaccharides from Burkholderia caryophylli.}, author={Antonio Molinaro and Cristina De Castro and Rosa Lanzetta and Antonio Evidente and Michelangelo Parrilli and Otto Holst}, journal={The Journal of biological chemistry}, year={2002}, volume={277 12}, pages={10058-63} }