Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri.

  title={Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri.},
  author={Renato Morona and Luisa Van den Bosch},
  journal={FEMS microbiology letters},
  volume={221 2},
Shigella flexneri 2a strain 2457T lipopolysaccharide (LPS) has O antigen (Oag) chains with two modal lengths (S-type and VL-type), and has IcsA apparently located at one pole on its cell surface. Treatment of Y serotype derivatives of 2457T and RMA696 (2457T wzz(SF)) with Sf6 tailspike protein (TSP) resulted in hydrolysis of Oag chains, and an increase in detection of IcsA by indirect immunofluorescence staining on both the lateral and polar regions of the cell surface. Newly synthesised IcsA… CONTINUE READING

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