Lipopolyamine-Mediated Single Nanoparticle Formation of Calf Thymus DNA Analyzed by Fluorescence Correlation Spectroscopy

@article{Adjimatera2006LipopolyamineMediatedSN,
  title={Lipopolyamine-Mediated Single Nanoparticle Formation of Calf Thymus DNA Analyzed by Fluorescence Correlation Spectroscopy},
  author={Noppadon Adjimatera and Teresa Kral and Martin Hof and Ian S. Blagbrough},
  journal={Pharmaceutical Research},
  year={2006},
  volume={23},
  pages={1564-1573}
}
PurposeThe aim of this study is to analyze linear calf thymus DNA (ct DNA) nanoparticle formation with N4,N9-dioleoylspermine and N1-cholesteryl spermine carbamate.MethodsFluorescence correlation spectroscopy (FCS) was used to determine the quality of ct DNA condensed by lipopolyamines. ct DNA was prelabeled with PicoGreen (PG) to allow fluorescence intensity fluctuation measurement and analysis.ResultsN4,N9-Dioleoylspermine efficiently condensed ct DNA into point-like molecules with diffusion… 
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