Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct.

@article{Wong1987LipoperoxidesIP,
  title={Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct.},
  author={S. Voon Wong and Joseph A. Knight and Sidney M. Hopfer and O Zaharia and Cathy Leach and F. William Sunderman},
  journal={Clinical chemistry},
  year={1987},
  volume={33 2 Pt 1},
  pages={214-20}
}
This assay of plasma lipoperoxides involves hydrolysis in dilute H3PO4 at 100 degrees C; complexation of malondialdehyde (MDA), a hydrolysis product, with thiobarbituric acid (TBA); methanol precipitation of plasma proteins; fractionation of the protein-free extract on a C18 column; and spectrophotometric quantification of the MDA-TBA adduct at 532 nm. The detection limit was 0.15 mumol of MDA per liter of plasma. Run-to-run precision (CV) averaged 8 to 13%. Analytical recovery of MDA after… CONTINUE READING
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