Lipid vesicle-mediated alterations of membrane cholesterol levels: Effects on Na+ and K+ currents in squid axon

@article{Steele1981LipidVA,
  title={Lipid vesicle-mediated alterations of membrane cholesterol levels: Effects on Na+ and K+ currents in squid axon},
  author={J. A. Steele and Mark J. Poznansky and Douglas C. Eaton and Malcolm S. Brodwick},
  journal={The Journal of Membrane Biology},
  year={1981},
  volume={63},
  pages={191-198}
}
We demonstrate that cholesterol can exchange from sonicated lipid vesicles to a perfused squid giant axon membrane and that vesicles with varying cholesterol/phospholipid (C/P) mole ratios can be used to achieve either net loading or net depletion of axon membrane cholesterol. Two types of evidence were obtained which show that net loading or depletion of… CONTINUE READING