Lipid synthesis and secretion by primary cultures of rat mammary epithelial cells.

@article{Rohlfs1993LipidSA,
  title={Lipid synthesis and secretion by primary cultures of rat mammary epithelial cells.},
  author={Elizabeth M. Rohlfs and Donald Louie and Steven H Zeisel},
  journal={Journal of cellular physiology},
  year={1993},
  volume={157 3},
  pages={469-80}
}
Lipid synthesis and secretion was measured in primary rat mammary epithelial cells cultured on basement matrix in medium supplemented with lactogenic hormones. The cells grew and differentiated to form alveolar-like structures reminiscent of lactating mammary gland. They synthesized abundant triacylglycerol, containing fatty acids characteristic of rat milk (C10:0-C14:0), using 14C-glucose, 14C-oleic acid or 14C-glycerol as precursors. Basal levels of triacylglycerol secretion were measured… CONTINUE READING