Lipid redistribution in phosphatidylserine-containing vesicles adsorbing on titania

@article{Khan2008LipidRI,
  title={Lipid redistribution in phosphatidylserine-containing vesicles adsorbing on titania},
  author={Tabrisur Rahman Khan and H. Michelle Grandin and Alireza Mashaghi and Marcus Textor and Erik Reimhult and Ilya Reviakine},
  journal={Biointerphases},
  year={2008},
  volume={3},
  pages={FA90-FA95}
}
Lipid vesicles (liposomes) exhibit a wide range of behavior at inorganic (oxide) surfaces. A complete understanding of the vesicle-surface interactions, and of the ensuing transformations surface adsorbed liposomes undergo, has proven elusive. This is at least in part due to the large number of degrees of freedom of the system comprising vesicles with their molecular constituents, substrate surface, and electrolyte solution. The least investigated among these degrees of freedom are those… Expand
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References

SHOWING 1-10 OF 75 REFERENCES
Lipid vesicle adsorption versus formation of planar bilayers on solid surfaces.
TLDR
The absorption and spreading behavior of lipid vesicles composed of either palmitoyloleoylphosphatidylcholine (POPC) or Escherichia coli lipid upon contact with a glass surface was examined, and supported planar bilayers were found to be permeable for small molecules, whereas supported vesicle layers were impermeable and thus represented immobilized, topologically separate compartments. Expand
Asymmetric distribution of phosphatidyl serine in supported phospholipid bilayers on titanium dioxide.
TLDR
It is shown that the mobility of the fluorescently labeled PS present in these bilayers is severely restricted, whereas that of the zwitterionic phosphatidyl choline is not affected, and it is proposed that, on TiO2, PS is trapped in the proximal leaflet of the bilayers. Expand
Surface-dependent transitions during self-assembly of phospholipid membranes on mica, silica, and glass.
TLDR
DOPC membranes on mica were rapidly degraded by phospholipase A2 (PLA2), which pleads against the role of membrane defects as initial PLA2 targets, in accordance with recent atomic force microscopy measurements of gel-phase phospholIPid degradation by PLA2. Expand
Effect of average phospholipid curvature on supported bilayer formation on glass by vesicle fusion.
TLDR
The results indicate that the average shape of lipid molecules within a vesicle (C(0,ave), described mathematically by the average intrinsic curvature, is essential for determining kinetically viable reactions that are responsible for global geometric changes. Expand
Vesicle adsorption and lipid bilayer formation on glass studied by atomic force microscopy.
TLDR
These studies were performed in part to unravel details of the previously observed isolated rupture of dye-labeled PC vesicles on glass, specifically to differentiate partial rupture, that is, pore formation and leakage of entrapped dye, from full rupture to form bilayer disks. Expand
Formation of Supported Phospholipid Bilayers from Unilamellar Vesicles Investigated by Atomic Force Microscopy
Since their introduction through the work of McConnell et al. in the early 80s, supported phospholipid bilayers (SPBs) have proven to be a versatile model system for investigating a wide variety ofExpand
Nanoporous microbead supported bilayers: stability, physical characterization, and incorporation of functional transmembrane proteins.
TLDR
The results of these experiments provide a basis for bulk ionic and fluorescent dye-based compartmentalization assays as well as single-molecule optical and single-channel electrochemical interrogation of transmembrane proteins in a biomimetic platform. Expand
Pathways of lipid vesicle deposition on solid surfaces: a combined QCM-D and AFM study.
TLDR
Calcium was shown to enhance the tendency of SLB formation for negatively charged and zwitterionic vesicles, and the role of vesicle-support, interbilayer, and intrabilayer interactions in the formation of SLBs is discussed. Expand
Interaction of a cationic polymer with negatively charged proteoliposomes.
TLDR
Proteoliposomes were prepared by making bilayer vesicles from neutral egg yolk lecithin and negatively charged alpha-chymotrypsin that had been previously stearoylated and these findings seem relevant to the effects of polyelectrolytes on cellular membranes. Expand
On the effect of the solid support on the interleaflet distribution of lipids in supported lipid bilayers.
TLDR
It is found that the underlying support can induce substantial redistribution of lipid material between the two leaflets and in particular, SLBs formed on mica showed a substantially depleted amount of accessible DOPS in the presence of calcium. Expand
...
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2
3
4
5
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