Lipid raft-associated protein sorting in exosomes.

@article{DeGassart2003LipidRP,
  title={Lipid raft-associated protein sorting in exosomes.},
  author={Aude De Gassart and Charles G{\'e}minard and Beno{\^i}t F{\'e}vrier and Graça Raposo and Michel Vidal},
  journal={Blood},
  year={2003},
  volume={102 13},
  pages={
          4336-44
        }
}
Exosomes are small membrane vesicles secreted by cells upon fusion of multivesicular endosomes with the cell surface. The mechanisms underlying the specific sorting of proteins in exosomal membranes are far from being unraveled. We demonstrate here, using different cells, that some molecules are released in the extracellular medium via their association with lipid raft domains of the exosomal membrane. Various typical raft-associated molecules could be detected by immunoblot in exosomes and… 

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References

SHOWING 1-10 OF 71 REFERENCES

Proteomic and Biochemical Analyses of Human B Cell-derived Exosomes

TLDR
Data suggest that recruitment of membrane proteins from the limiting membranes into the internal vesicles of multivesicular bodies may involve their incorporation into tetraspanin-containing detergent-resistant membrane domains.

The exosome pathway in K562 cells is regulated by Rab11.

TLDR
The results demonstrate that in K562 cells Rab11 modulates the exosome pathway although the exact step involved is still not known.

Selective Enrichment of Tetraspan Proteins on the Internal Vesicles of Multivesicular Endosomes and on Exosomes Secreted by Human B-lymphocytes*

TLDR
Exosomes are enriched in the co-stimulatory molecule CD86 and in several tetraspan proteins, including CD37, CD53, CD63, CD81, and CD82, and Interestingly, subcellular localization of these molecules revealed that they were concentrated on the internal membranes of multivesicular MIICs.

Proteomic Analysis of Dendritic Cell-Derived Exosomes: A Secreted Subcellular Compartment Distinct from Apoptotic Vesicles1

TLDR
Using a systematic proteomic approach, the first extensive protein map of a particular exosome population is established and a novel category of exosomal proteins related to apoptosis is identified: thioredoxin peroxidase II, Alix, 14-3-3, and galectin-3.

B lymphocytes secrete antigen-presenting vesicles

TLDR
It is demonstrated by immunoelectron microscopy that the limiting membrane of MIICs can fuse directly with the plasma membrane, resulting in release from the cells of internal MHC class II-containing vesicles, suggesting a role for exosomes in antigen presentation in vivo.

Lipid Domain Structure of the Plasma Membrane Revealed by Patching of Membrane Components

TLDR
The data strongly suggest that coalescence of cross-linked raft elements is mediated by their common lipid environments, whereas separation of raft and non-raft patches is caused by the immiscibility of different lipid phases, supported by the finding that cholesterol depletion abrogated segregation.

Exosomes released during reticulocyte maturation bind to fibronectin via integrin α4β1

TLDR
It is shown here that integrin alpha4beta1, present on the surface of erythroid precursors but absent from the mature red cell membrane, is at least partly cleared from the reticulocyte plasma membrane by the exosomal pathway.

The Biogenesis and Functions of Exosomes

Exosomes are membrane vesicles with a diameter of 40–100 nm that are secreted by many cell types into the extracellular milieu. They correspond to the internal vesicles of an endosomal compartment,

Exosomes released during reticulocyte maturation bind to fibronectin via integrin alpha4beta1.

TLDR
It is shown here that integrin alpha4beta1, present on the surface of erythroid precursors but absent from the mature red cell membrane, is at least partly cleared from the reticulocyte plasma membrane by the exosomal pathway.

Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts.

TLDR
Stomatin and the flotillins are present as independently organized high-order oligomers, suggesting that these complexes act as separate scaffolding components at the cytoplasmic face of erythrocyte lipid rafts.
...