Lipid peroxidation and the thiobarbituric acid assay: standardization of the assay when using saturated and unsaturated fatty acids.

@article{Rael2004LipidPA,
  title={Lipid peroxidation and the thiobarbituric acid assay: standardization of the assay when using saturated and unsaturated fatty acids.},
  author={Leonard T Rael and Gregory W. Thomas and Michael L Craun and C. Gerald Curtis and Raphael Bar-Or and David Bar-Or},
  journal={Journal of biochemistry and molecular biology},
  year={2004},
  volume={37 6},
  pages={749-52}
}
Saturated fatty acids are less vulnerable to lipid peroxidation than their unsaturated counterparts. In this investigation, individual fatty acids of the C(16), C(18) and (20) families were subjected to the thiobarbituric (TBA) assay. These fatty acids were chosen based on their degree of saturation and configuration of double bonds. Interestingly, an assay threshold was reached where increasing the fatty acid concentration resulted in no additional decrease in the TBARS concentrations… CONTINUE READING
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