Lipid-mediated impairment of normal energy metabolism in the isolated perfused diabetic rat heart studied by phosphorus-31 NMR and chemical extraction.

@article{Pieper1984LipidmediatedIO,
  title={Lipid-mediated impairment of normal energy metabolism in the isolated perfused diabetic rat heart studied by phosphorus-31 NMR and chemical extraction.},
  author={Galen M. Pieper and J. Mitchell Salhany and Wallace Jennings Murray and Shao T. Wu and Robert S. Eliot},
  journal={Biochimica et biophysica acta},
  year={1984},
  volume={803 4},
  pages={
          229-40
        }
}
The relationship between extracellular palmitate and the accumulation of long-chain fatty-acyl coenzyme A with that of high-energy phosphate metabolism was investigated in the isolated perfused diabetic rat heart. Hearts were perfused with a glucose/albumin buffer supplemented with 0, 0.5, 1.2 or 2.0 mM palmitate. 31P-NMR was used to analyze phosphocreatine and ATP metabolism during 1 h of constant-flow recirculation perfusion. At the end of perfusion, frozen samples were taken for chemical… CONTINUE READING