Lipid droplets are novel sites of N-acylethanolamine inactivation by fatty acid amide hydrolase-2.

@article{Kaczocha2010LipidDA,
  title={Lipid droplets are novel sites of N-acylethanolamine inactivation by fatty acid amide hydrolase-2.},
  author={Martin Kaczocha and Sherrye T. Glaser and Janiper Chae and Deborah A. Brown and Dale G. Deutsch},
  journal={The Journal of biological chemistry},
  year={2010},
  volume={285 4},
  pages={2796-806}
}
Anandamide (AEA) and other bioactive N-acylethanolamines (NAEs) are primarily inactivated by the enzyme fatty acid amide hydrolase (FAAH). Recently, FAAH-2 was discovered in humans, suggesting an additional enzyme can mediate NAE inactivation in higher mammals. Here, we performed a biochemical characterization of FAAH-2 and explored its capacity to hydrolyze NAEs in cells. In homogenate activity assays, FAAH-2 hydrolyzed AEA and palmitoylethanolamide (PEA) with activities approximately 6 and… CONTINUE READING