Linker histone subtypes differ in their effect on nucleosomal spacing in vivo.

@article{berg2012LinkerHS,
  title={Linker histone subtypes differ in their effect on nucleosomal spacing in vivo.},
  author={Christine {\"O}berg and Annalisa Izzo and Robert Vincent Schneider and {\"O}rjan Wrange and Sergey Belikov},
  journal={Journal of molecular biology},
  year={2012},
  volume={419 3-4},
  pages={
          183-97
        }
}
Linker histone H1 is located on the surface of the nucleosome where it interacts with the linker DNA region and stabilizes the 30-nm chromatin fiber. Vertebrates have several different, relatively conserved subtypes of H1; however, the functional reason for this is unclear. We have previously shown that H1 can be reconstituted in Xenopus oocytes, cells that lack somatic H1, by cytosolic mRNA injection and incorporated into in vivo assembled chromatin. Using this assay, we have expressed… CONTINUE READING
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