Linker histone protection of chromatosomes reconstituted on 5S rDNA from Xenopus borealis:a reinvestigation.

@article{An1998LinkerHP,
  title={Linker histone protection of chromatosomes reconstituted on 5S rDNA from Xenopus borealis:a reinvestigation.},
  author={Woojin An and Kensal E van Holde and Jordanka S. Zlatanova},
  journal={Nucleic acids research},
  year={1998},
  volume={26 17},
  pages={4042-6}
}
The location of the linker histone (LH) on the nucleosome has been the subject of recent controversy. Although previous evidence had supported a location over the dyad axis, some recent experiments suggest an asymmetric, off-axis position. In this paper we show that the DNA sequence used to reconstitute chromatosomes in these experiments is prone to artifacts in nuclease digestion: results interpreted as 'protection' by LHs can be obtained with either naked DNA or with reconstituted core… CONTINUE READING