Linkage-specific conformational ensembles of non-canonical polyubiquitin chains.

@article{Castaeda2016LinkagespecificCE,
  title={Linkage-specific conformational ensembles of non-canonical polyubiquitin chains.},
  author={Carlos A. Casta{\~n}eda and Apurva Chaturvedi and Christina M. Camara and Joseph E. Curtis and Susan Krueger and David Fushman},
  journal={Physical chemistry chemical physics : PCCP},
  year={2016},
  volume={18 8},
  pages={
          5771-88
        }
}
Polyubiquitination is a critical protein post-translational modification involved in a variety of processes in eukaryotic cells. The molecular basis for selective recognition of the polyubiquitin signals by cellular receptors is determined by the conformations polyubiquitin chains adopt; this has been demonstrated for K48- and K63-linked chains. Recent studies of the so-called non-canonical chains (linked via K6, K11, K27, K29, or K33) suggest they play important regulatory roles in growth… 
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45 Citations

Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins.

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In vivo modulation of ubiquitin chains by N-methylated non-proteinogenic cyclic peptides

Small, non-proteinogenic cyclic peptides, rich in non-canonical features like N-methylation, which can tightly and specifically bind Lys48-linked Ub chains are discovered and highlighted as a promising anti-cancer mechanism of action.

Branching via K11 and K48 Bestows Ubiquitin Chains with a Unique Interdomain Interface and Enhanced Affinity for Proteasomal Subunit Rpn1.

Role of Polyubiquitin Chain Flexibility in the Catalytic Mechanism of Cullin-RING Ubiquitin Ligases.

Cullin-RING ubiquitin ligases are a diverse family of ubiquitin ligases that catalyze the synthesis of K48-linked polyubiquitin (polyUb) chains on a variety of substrates, ultimately leading to their…

Towards a molecular basis of ubiquitin signaling: A dual-scale simulation study of ubiquitin dimers

This work has combined exhaustive coarse grained and atomistic simulations of all eight possible ubiquitin dimers with a suitable dimensionality reduction technique and a new method to characterize protein-protein interfaces and the conformational landscape of protein conjugates and found that ubiquitous dimers exhibit characteristic linkage type-dependent properties in solution, such as interface stability and the character of contacts between the subunits.

Mechanistic insights into the enhancement or inhibition of phase separation by polyubiquitin chains of different lengths or linkages

This study provides mechanistic insights into how the structural and conformational properties of polyubiquitin chains contribute to heterotypic phase separation with ubiquitin-binding shuttles and adaptors.

The evolving world of ubiquitin: transformed polyubiquitin chains

These novel forms of ubiquitin signal are reviewed, with a focus on fibril formation of polyubiquitin chains and its underlying biological relevance.

Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry.

The complimentary nature of linkage-specific antibodies and mass spectrometry proteomics for the characterization of complex ubiquitin signals using lessons learned in early development of both technologies are described.

Chemical ubiquitination for decrypting a cellular code

The aim of this review is to provide an overview of the techniques that are available for preparing Ub conjugates and polyUb chains with focus on approaches that use recombinant protein building blocks.

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