Linkage of a triple helix-forming oligonucleotide to amsacrine-4-carboxamide derivatives modulates the sequence-selectivity of topoisomerase II-mediated DNA cleavage.

@article{Arimondo2000LinkageOA,
  title={Linkage of a triple helix-forming oligonucleotide to amsacrine-4-carboxamide derivatives modulates the sequence-selectivity of topoisomerase II-mediated DNA cleavage.},
  author={Paola B. Arimondo and Christian Bailly and Alexandre S Boutorine and Ulysse Asseline and J S Sun and Th{\'e}r{\`e}se Garestier and Claude H{\'e}l{\`e}ne},
  journal={Nucleosides, nucleotides & nucleic acids},
  year={2000},
  volume={19 8},
  pages={1205-18}
}
Amsacrine-4-carboxamide-oligonucleotide conjugates were synthesized and studied for their capacity to form DNA triple helices and to alter human topoisomerase II binding and cleavage properties. The intercalating agent was attached to the 3'- or the 5'-end of a 24 nt triple helix-forming oligonucleotide via linkers of different lengths. The stability of these DNA triple helices was investigated by gel retardation and melting temperature studies using a synthetic 70 bp DNA duplex target. The… CONTINUE READING