Linkage mapping by simultaneous screening of multiple polymorphic loci using Alu oligonucleotide-directed PCR.

@article{Zietkiewicz1992LinkageMB,
  title={Linkage mapping by simultaneous screening of multiple polymorphic loci using Alu oligonucleotide-directed PCR.},
  author={Ewa Zietkiewicz and Marcin Labuda and Daniel Sinnett and Francis H Glorieux and Damian Labuda},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 18},
  pages={8448-51}
}
We present the use of our recently described multiple-loci polymorphic DNA markers ("alumorphs") for linkage mapping of the human genome. By using the polymerase chain reaction (PCR) with an Alu-specific primer we could reveal, in a single experiment, up to 20 genomic polymorphisms seen as the presence or absence of amplified DNA fragments originating from genomic segments flanked by Alu repeats. Using this approach we examined genomic DNA samples from two families with a history of… CONTINUE READING