Linkage between proton binding and amidase activity in human alpha-thrombin: effect of ions and temperature.

@article{Cera1991LinkageBP,
  title={Linkage between proton binding and amidase activity in human alpha-thrombin: effect of ions and temperature.},
  author={Enrico Di Cera and Raimondo de Cristofaro and David J. Albright and John W. Fenton},
  journal={Biochemistry},
  year={1991},
  volume={30 32},
  pages={7913-24}
}
The amidase activity of human alpha-thrombin has been studied at steady state in the pH range 6-10, as a function of NaCl concentration from 1 mM to 1 M and temperature from 10 to 40 degrees C. The Michaelis-Menten constant, Km, shows a bell-shaped dependence over this pH range with a minimum around pH 7.5 in the presence of 0.1 M NaCl at 25 degrees C. The catalytic constant, kcat, also has a bell-shaped pH dependence with multiple inflection points that are more evident at low NaCl… CONTINUE READING

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